Diagnosis of tick-borne encephalitis and borreliosis by blood

encephalitis mites that live in the grass in the meadows, in the woods are vectors of serious human diseases for: tick-borne encephalitis, Lyme disease. The encephalitis virus acts directly on the central nervous system, and if the treatment is not taken on time, then a lethal outcome is possible in a week.

sad statistics says that one-third of infected tick-borne encephalitis die if treatment was not carried out any later. Therefore, timely diagnosis and treatment initiation are extremely important. The leading role in the diagnosis of tick-borne encephalitis is analyzed by encephalitis.

Who needs to spend

analysis of the need to conduct analysis on encephalitis and Lyme disease occurs in the following cases:

• if a person is sure that he was bitten by a tick or suspects it;
• if the terrain is epidemic in this disease;
• if there are clinical symptoms of tick-borne encephalitis infection and laboratory confirmation is necessary.

The following assays are used:

• PCR( polymer chain reaction);
• ELISA( enzyme immunoassay);
• Western blotting;
• analysis for the presence of a virus in a tick.

Do you know that the causative agent of cerebral viral encephalitis is an arbovirus of the genus Flavivirus?

What is viral encephalitis can be read here.

IFA IFA - a reliable and informative in the diagnosis of tick-borne encephalitis, a blood test for Lyme disease and encephalitis, revealing immunnoglobulinovye proteins. If a person is ill with tick-borne encephalitis, the analysis shows the presence in his blood of specific antibodies, the elaboration of which the body reacts to the penetration into the human body tick-borne encephalitis virus. Such elements are specific protein complexes M and G.

Unfortunately such a protective reaction for self-healing is not enough for the body. More scale development of antibodies is observed only 3-4 weeks after the bite. But after such terms the treatment can become already useless. Drug therapy is mandatory and begins without delay. Therefore, with a positive result, the patient is hospitalized in the hospital in urgent order.

During the first 24 hours after infection, this analysis may be negative. It takes some time for the virus to spread, begin to exert its pathogenic effect, was detected by the immune system, which will trigger the production of proteins G and M. Before both proteins can not be determined for a while. But if they are identified, we can confidently say that the tick-borne encephalitis and Lyme disease is actually present in the blood and the disease is in an acute phase.

Important! A situation is possible when IgG is detected, and IgM is absent. This suggests that tick-borne encephalitis has already been in the past in this person, or he is vaccinated from it. The positive result is preserved for 12 days. Reliable is the result obtained after several times of blood testing:

• for the 1st time, after having bitten the tick on the fifth, seventh day;
• 2 - two months later;
• 3 - in six months.

Western blotting and PCR

Western blotting - this method is relevant if the assay for detecting immunoglobulins is doubtful or little time has passed from the bite. This method, Western blotting, a protein immunoblot allows us to identify specific immunoglobulins, which are produced by the body for a dozen antigens, characteristic of certain pathogens carried by ticks.

For the PCR method, the patient's blood or his cerebrospinal fluid is used. The analysis of blood on encephalitis allows to determine the DNA and RNA of the virus. This method of blood analysis is especially important in the early stages of the disease, when the definition of antibodies is not yet very informative. The analysis allows to detect both active virus and inactivated pathogens.

Analysis of PCR of cerebrospinal fluid requires spinal cord puncture and collection of cerebrospinal fluid. It should be performed only in aseptic conditions that are possible in the hospital, as well as the professional skills that anesthesiologists possess. This manipulation has certain contraindications, so its use is not always possible.

Important! In addition, that the cerebrospinal fluid is examined for the presence of an agent, it is checked for leukocyte count and protein level, which allows to judge the inflammatory process taking place in the central nervous system.

It is also possible to study the cerebrospinal fluid to determine the culture of the virus. So it is identified which tick-borne encephalitis virus became the cause of the disease. To do this, the obtained cerebrospinal fluid is placed in a special medium favorable for reproduction of the encephalitis virus, on which its growth is determined after a time, if it was in the cerebrospinal fluid under investigation. In order to observe the growth of the virus it will take at least a week.

Important! The results of the analyzes enable the specialist to judge not only the presence of the disease, but also its stage.

There is also a definition of PCR from a tick. For this tick( if a person is sure that this tick was bitten by him), put it in a glass tube and deliver it to the laboratory.

What should I do to treat viral encephalitis? Prophylaxis of the disease.

Do you know that with viral encephalitis there is Parkinsonism? All about the symptoms of the pathology here.

All about encephalitis is available at this address http: //golmozg.ru/zabolevanie/ encefalit-klassifikaciya.html. Causes, types, symptoms.

Preparing and conducting a blood test

How to prepare and where to pass the analysis for encephalitis? Preparation, like the blood sampling itself, is simple. The last meal before taking blood should be dinner, and no less than 8 hours before the study. It is also necessary to give up smoking at the same time. The patient's blood is taken from the vein in the morning. You can take tests in the centers of laboratory diagnostics, infectious hospitals.

Timely diagnosed tick-borne encephalitis and competent treatment can cure it once and for all without consequences for the human body. If it is necessary to conduct a blood test for encephalitis and borreliosis, its price is about 2000 rubles. On both diseases.