Urine boiling: rules of preparation for delivery and interpretation of analyzes
Bacterial culture of urine is a laboratory diagnostic method that allows to determine the presence of microorganisms, as well as their quantity in urine.The research is often prescribed by doctors.So, it is carried out with suspicion of infectious diseases of the urinary system.In addition, bacterial culture of urine is mandatory made pregnant.Table of contents: Preparing for the study Indications for the study Study procedure Interpretation of results Bacteriuria in pregnant women
Preparing for the study
For the results of the study to be reliable, urine collection must be properly performed.It is recommended to urinate in the morning after waking up.First of all, you need to hold the toilet of the external genital organs without using soap, intimate gels and other antiseptic drugs.Then it is necessary to soak the perineum with a clean towel.During menstruation, doctors recommend that women use tampons when collecting urine for analysis.
To prevent distortion of results, the dishes used to store urine must be sterile.In the Soviet era, a person before the study had to go to a medical institution, where he was given a sterile jar.Fortunately, now everything is much easier: just go to the nearest pharmacy and buy a container for urine.
For bacterial examination, it is necessary to collect an average portion of urine in the container.This means that the first portion of urine descends into the toilet, then it is necessary to delay urination, bring the container and fill with urine.The filled container can be closed with a lid and set aside.The final portion of urine should be drained into the toilet.
Important: The must not be touched with the fingers of the inner surface of the container.This can be avoided by keeping the container directly at the base.
The quality of the result is influenced by the timing of the study.Therefore, urine should be brought to the laboratory within two to three hours.
Indications for the urine culture tank
The urine culture tank is performed in such cases:
- If there is a suspicion of an inflammatory process in the urinary organs;
- To control the effectiveness of antibiotic treatment of diseases of the urinary system;
- During pregnancy.
In addition, carrying out a urine culture tank together with an antibioticogram will determine the most effective antibacterial drug for treating infection.
Technique for performing a urine culture tank
The laboratory assistant applies urine samples to special nutrient media placed in Petri dishes.The media are placed in thermostats, where optimal conditions for the growth of microorganisms are created.At certain intervals, the lab technician examines the nutrient media.The growth of microorganisms occurs in the form of colonies.The concentration of pathogenic microorganisms is measured in CFU-colony-forming units.That is, this is the number of colonies that will grow from one cell.
The normal microflora of the urinary tract includes staphylococci( St. epidermidis) , streptococci( Str. Faecalis) . For infections of the urinary system in the urine are determined:
- Intestinal bacillus( Escherichia coli ) ;
- Saprophytic Staphylococcus aureus( Staphylococcus saprophyticus);
- Pseudomonas aeruginosa( Pseudomonas aeruginosa);
- Proteus( Proteus mirabilis);
- Klebsiels( Klebsiella pneumonia ).
Explanation of the results of bacterial urine
Human urine is not sterile, so the isolation of microorganisms in small concentrations( less than 103 microbial cells per ml) is the norm.Bacteriuria, equal to 104 microbial cells per ml, is assessed as a questionable result.More than 105 microbial cells in 1 ml indicates the presence of infection in the urethra.The results of bacterial culture of urine a person can receive in five to ten days.
This diagnostic method allows you to determine the presence of an infectious process in the genitourinary system, but does not provide information about which organ the inflammatory process is located in.To understand this will help the results of a three-glass urine sample.
Bacteriuria in pregnant women
As soon as a pregnant woman becomes registered in a women's consultation, she is immediately prescribed a bunch of directions for the passage of all sorts of research, including bacterial culture of urine.Then the pot is sown in the urine of pregnant women repeated at the 36th week of pregnancy.
Bacterial culture of urine will reveal a pregnant woman with pyelonephritis.Anatomical features of the genitourinary system of the female body explain the predisposition to the development of pyelonephritis.So, many women have an infectious process in the kidneys, which, however, they may not even guess.During pregnancy, the infectious process is activated, which is a danger not only for women, but also for the child.
Pyelonephritis in pregnant women develops due to the following reasons:
- Reducing the tone of the ureters, which leads to stagnation of urine and multiplication of bacteria;
- The enlarged uterus compresses the urinary tract, which also leads to stagnation of urine.
Pregnancy pyelonephritis occurs in approximately 3-10% of all cases.It is because of such a high prevalence of the disease that everyone carries out a urine culture tank for pregnant women.
It should be noted that with a single culture of urine, false positive results often occur.Therefore, all pregnant women with positive results, the seeding tank should repeat the test after one or two weeks, paying close attention to the careful toilet of the external genitalia before taking the urine.
Grigorova Valeria, medical reviewer