Bacteriological sowings ejaculate: how and why to give up

What is sown on the microflora?

Sowing on flora, he's a tank crop, he is sowing to antibiotics - a laboratory study, by means of which You can determine the type of infectious agent, its concentration in the body, as well as resistance to drugs substances. These data allow physicians to accurately diagnose disease and plan the most efficient treatment strategy.

Bacteriological sowing can be carried out using various types of substrate.

Depending on the intended diseases and organ damage, as the material can be used for virtually any biological studies liquid blood, ejaculate, allocating different mucous membranes, cerebrospinal fluid and articular contents pathological formations (abscesses, cysts), urine, as well as etc.

Bakposev consists in seeding of bacteria from a sample (diagnostic material) into the culture medium, followed by observation of the quantitative changes of bacterial colonies. Calculus results determined in CFU - colony-forming units, ie, amount of viable bacterial cells, capable of forming new colonies.

Where to use the method of sowing on the microflora?

In accordance with a different material for the study, the results can be detected by various types of pathogens. For example, seeding feces can show groups such bacteria as Salmonella, Shigella, tifoparatifoznye bacteria, anaerobic bacteria.

seeding feces often dysbacteriosis with the determination of sensitivity to antibiotics administered to patients who are experiencing gastrointestinal disorders after long-term use of oral antibiotics.

Deciphering this analysis allows to distinguish between infectious intestinal disorders by dysbiosis.

The sexually transmitted infection, gynecological, urological practice using analysis of urogenital mucus bacterial seeding. In this form of the analyte can be detected, in addition to bacteria, fungi.

Among bacterial groups are most likely to detect gookokki, Listeria, ureaplasma, and is suspected a particular sexual infection your doctor may prescribe seeding for mycoplasma or other bacteriological culture.

Often in pulmonology and general therapeutic practices performed sputum on flora and sensitivity to antibiotics. This analysis allows us to establish the true cause bronchitis, and share infectious form of allergic and other. As an analyte used with detachable expectoration.

Such fluids, breast milk, blood, urine, bile, joint fluid by nature, are sterile, so for them is carried out on the crop contamination, ie to identify any forms of bacteria, as in any case they will be pathological.

We should also mention the planting of ejaculate, or, in other words, sperm flora. Sowing of sperm used in urological practice and allows you to effectively identify genital infections. It can be used as seed in the contamination of the ejaculate for flora and the presence of a specific analysis pathogen - such as crop on a chlamydia with the definition of bacteriological sensitivity antibiotics.

How is the diagnostic process?

After collection of diagnostic material flora and sensitivity to antibiotics, its "sown" in various culture media. The choice of medium is determined by the capabilities of the particular diagnostic laboratory and diagnostic purposes.

For example, to determine the presence of a particular pathogen is used most suitable for his environment and to distinguish between different pathogens - differential diagnostic medium.

For identification of different bacterial colonies using a solid medium.

After seeding product flora susceptibility to antibiotics, the substrate is placed in a thermostat, which functions with the set values ​​of the temperature, humidity and time.

Thereafter, the obtained colonies were recovered antibiotics, stained produce count control and inspection.

If necessary, quantification of the bacteria, the substrate is repeatedly diluted and then assesses defined culture (or culture) of the bacteria as a whole.

Often, patients are wondering why the implementation of planting on the flora with the definition of sensitivity to advanced spectrum antibiotics in the lab takes a long time (up to 10 days, for example, in determining the blood sterility).

This is due to the specific growth of bacteria: the process of maturation in the thermostat can not be accelerated without the risk of the death of the colony.

In addition, if you want to carry out a quantitative analysis of several types of bacteria, it takes a lot of time in connection with the processing of quantitative results.

How to collect material for analysis?

Requirements to patients who donate material for analysis is as high as to the technicians in the medical facility. Seeding at identifying bacteria requires great care with regard to the test material, as in the case of non-compliance Sterility will colonization by bacteria that have no clinical significance and an analysis result will be spoiled.

Therefore, the first requirement - is the use of specialized sterile utensils.

The second - is that the material is diagnosed if it is for some reasons going beyond the laboratory, it is necessary to deliver as soon as possible.

The fresher be investigated medium, the higher the chance to get clinically correct result of the study.

This rule provides priority fence material directly in the laboratory, or in close proximity thereto.

Third - this is what the staff, collecting material for analysis, must possess certain skills. This applies to the two preceding paragraphs: maintaining sterility of the collected material and its rate of sending to the study. Besides, the sowing and counting CFU can only be done by highly qualified personnel.

Requirements for collecting diagnostic material

1. Collecting urine analysis should be carried out in the morning, after the hygiene of genitals procedures in a sterile container. Collecting subject first morning urine sample, which shall be from 10 to 15 ml. Donating material need not later than 2 hours after its preparation.
2. stool sampling is carried out in the morning, without the use of enemas and laxatives, sterile spatula into a sterile container. The volume of the collected material should be 15-30 g Donating cal possible within 5 hours of collection, it does not permit its cooling or freezing, because it can lead to death of the bacteria.
3. In the analysis of the destination on a smear from the nasal and mouth rinse is not allowed these mucous antibacterial solutions, drinking, eating, teeth not be cleaned prior to sampling analysis.

Diagnosed material immediately after collection in the laboratory goes to trial.

On the contrary, if the patient shows sputum, it will need to brush your teeth and rinse your mouth regularly antibacterial solution before delivery of the material.

When checking the blood for sterility is not allowed pre-antibiotics. Material collected in the laboratory, in compliance with the rules of sterility.

Breast milk also can be assembled at home or in the ward.

For this we first conducted aqueous hygiene procedures, and then the skin around the teat is treated with a disinfectant solution, and the milk is decanted into a sterile container. Deliver it to the laboratory need to 2 hours.

Smear genitals to women held in the middle of the menstrual cycle (no earlier than 14 days after completion of menstruation). In this case the sample to women can not urinate 2 hours men - about 5-6 hours. Sampling is carried out by your doctor. Similarly, sperm collection must be conducted under sterile conditions.

Results planting on the flora

In identifying a particular bacterial culture normal response will be a result that can be labeled with the words "no growth" or "sample results are negative."

If it was determined the microflora of a substance as a whole, a good sign is the designation of "normal".

Sensitivity to antibiotics appears with the study medication and the words "yes" and "no" to indicate the presence or absence of the effectiveness of a particular drug.

When expressed in bacteria CFU values, parameters must distinguish pathogenic and conditionally pathogenic flora. The latter can vary quite widely in their performance, without causing any harm to the human body.

Under normal rates near quantitative value indicated by the word "norm". In the case of pathogenic flora, "normal" loses its meaning, since

pathogenic organisms, in principle, should not be found in a healthy body, and the value of CFU then becomes important.

Anyway, trust transcript obtained results better than your doctor, as he in exactly know what the purpose pursued diagnosis and how to further dispose of these results.

In general, it should be noted that the planting on the flora is one of the most accurate, though time-consuming, laboratory The study, which allows you to diagnose and predict disease and develop effective its tactics treatment.

A source: https://proantibiotik.ru/vzroslym/posev-na-floru-s-opredeleniem-chuvstvitelnosti-k-antibiotikam

How is and what seed semen on antibiotics?

Those who intend to be screened for infertility to identify the causes, of course, are interested in what they are to what tests. The first method is the study of the disease - a semen analysis. Also, patients are likely to be asked to deliver the semen to and bakposev. So what is the sperm seed and what is it for?

When and how to perform the sperm bakposev

Bacteriological or semen tank crop on flora is one of the important diagnostic methods.

Basically it is assigned when the man revealed inflammation in the genitourinary system - for example, in the testicles or prostate; this feature is the increase in the number of leucocytes in semen analysis. Assign it and the diagnosis of sexually transmitted infections.

What brings bakposev?

bacterial inoculation test results to determine the presence of microorganisms such as mycoplasma, chlamydia, ureaplasma. It gives an idea of ​​the conditionally pathogenic flora (ie potentially dangerous), the representatives of which are streptococci, staphylococci, enterococci and other bacteria.

These strains are able to penetrate into the gonads and generalized inflammation, promoting change in the structure of the seminal vesicles, testes, prostate, and other organs.

This study is called semen culture antibiotics, because it reveals the sensitivity of detection microorganisms to antibiotics and thus determine the most effective antibiotics to combat pathogenic microflora.

How shall sperm on bakposev?

Finding out what crop sperm, nice to know, and on the order of analysis. In order to be able to explore a "clean" material, the patient should stop taking antibiotics for at least two weeks before the event. Encouraged abstinence from alcohol and sexual activity at least 4-5 days - as well as in the semen analysis.

The ejaculate must be given to the study as soon as possible. Most likely, the patient will be asked to pass it directly in the clinic. Some institutions allow to deliver the sperm out of the house, always in a sterile container.

And another tip: choosing a doctor, a urologist or andrologist, ask and facilities, which are available at the clinic. Unfortunately, some of them still offer to hand over such a delicate analysis of the toilet. But the presence of a specially equipped room will certainly help the patient feel more comfortable and fully trust experts.

A source: http://urolog.pw/st/chto-takoe-posev-spermy

Bacteriological sowing (bakposev)

Bacteriological sowing (bakposev) - a microbiological laboratory study human biological material through its sowing certain culture media at a certain temperature condition to determine the presence therein of any amount of pathogenic and conditionally pathogenic microorganisms and further tasks specific solutions treatment.

When allocating certain microorganisms is carried out a second important Analysis - antibiotikogrammy - determination of the sensitivity of detection of pathogens to antibiotics and bacteriophages.

The advantages of bacteriological seeding are:

• The high specificity of the method (ie, cross false reactions not observed). • The ability to explore absolutely any human biological fluid.
• Therapeutic goal - the definition of the sensitivity of detection of the microbe to a particular therapeutic agent (antibiogram), making it a high enough precision to conduct therapeutic purposes.

Disadvantages of bacteriological seeding:

• Duration obtain a result. • High requirements for the fence material.
• Specific requirements for qualification of personnel bacteriological laboratories.

Indications for bacteriological examination

The use of microbiological research method is a common medical practice, in particular infectious diseases, gynecology, urology, surgery, otolaryngology, oncology and others. Unconditional indication for the need for any bacterial inoculation test is an inflammatory disease of human organs and body systems, suspicion of a septic process.

The material for the bacterial inoculation test

To study the climb following the biological environment of the human body: nasopharyngeal mucus, mucus from the throat, the secret of the bronchial tree (sputum), stool (feces) urethra mucus, cervical, prostate secretion, urine, blood, cerebrospinal fluid, breast milk, bile, the contents of cysts, inflammatory lesions, wound discharge.

Which microorganisms can be identified by bakposeve

In the nose and throat mucus can detect hemolytic streptococci (Streptococcuc pyogenes, Streptococcuc agalactiae), pneumococci (Streptococcuc pneumoniae), Staphylococcus aureus (Staphylococcus auereus), korinobakterii diphtheria (Corynebacterium diphtheriae), Haemophilus influenzae (Haemophilus influenzae type b), meningococci (Neisseria meningitidis), Listeria (Listeria).

In trying to identify the faeces intestinal bacteria group - shigella and salmonella (Salmonella spp, Shigella spp..) Yersinia (Iersiniae. Spp), typhoid, paratyphoid group of bacteria (Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B), conventionally - pathogenic activators of intestinal infections, anaerobic microbes, pathogens of food poisoning, as well as to examine the feces on a dysbacteriosis bowel.

The contents of wounds biopunktate, purulent can identify Pseudomonas aeruginosa or Pseudomonas (Pseudomonas aeruginosa).

Mucus urogenital tract examined for the presence in it of pathogens, sexually transmitted diseases - gonorrhea, trichomonas, fungi (Neisseria gonorrhoeae, Trichomonas vaginalis, fungi of the genus Candida), ureaplasma (Ureaplasma urealyticum), mycoplasma (Mycoplasma hominis), Listeria (Listeria), you can also explore the smear on bacterial flora.

Blood can sow (explore) for sterility.

Materials such as breast milk, urine, prostatic, scraping, smear, wound contents, joint fluid, bile assayed for total contamination (bacterial flora).

What is the bacteriological seeding?

• Material for the study in the bacteriological laboratory placed (do seeding) on ​​special culture media. Depending on the desired search of a pathogen or group of pathogens of crop produced on various media.
• For example, it may be selective or elective culture medium (for growth of a single pathogen, growth of other microbes thus inhibited) Example which can be folded horse serum for detection of pathogens or diphtheria medium with selenite or bile salts for detecting intestinal pathogens.
• Another example may be a differential diagnostic medium (Hiss medium) that is used to decrypt the bacterial cultures. If necessary, liquid media do subcultured on solid medium with the aim of identifying the most colonies.
• Then the culture medium was placed in a thermostat (special device), in which conditions are favorable (Temperature, humidity, etc.) For growth and multiplication of pathogens in a thermostat medium are determined time.

Further conduct visual inspection of colonies of microorganisms which are called "microorganism culture". If necessary, a material microscopy colonies provisional special dyes.

What is evaluated at the control examination? This shape, color, density colonies, after further research - the ability to degrade some inorganic and organic compounds.

Further carried count pathogens.

In the microbiological investigation accounted for such a thing as colony forming unit (CFU) - a microbial cell capable of forming a colony, or a colony of microbes visible.

By CFU possible to determine the concentration or amount of microorganisms in the sample. CFU count is conducted by various methods: by counting the colonies under the microscope, the method of serial dilutions, the sector method.

Terms sampling biological material for bacteriological seeding

Bacteriological quality of the crop depends on the correctness of the collection of material for the study.

You need to remember a simple rule: sterile glassware and sterile instruments! Failure to comply with these requirements will lead to contamination (contamination of the outer skin material and representatives mucous membranes, the environment, do not have clinical significance), which will automatically make a study meaningless. To collect the material using sterile container, which is issued in the most bacteriological laboratory to the patient on his hands during outpatient examination for sampling the feces and urine. Of the various foci of inflammation fence performed only sterile instruments (spatulas, loops, spoons) specially trained health care professional (usually in the clinic nurse or infectious sight offices).

Blood and urine are collected in dry tubes, other materials in a container to transport a nutrient medium.

Another rule: fence material before the start of antibiotic therapy! In patients receiving antibiotics result will be significantly distorted. If you take these drugs, then stop taking them 10 days before the study and inform your doctor about the fact of receipt of any antibacterial drugs.

It should be provided with fast delivery to the lab! Microorganisms can be lost during drying, the change of acidity. For example, feces must be delivered in the form of heat.

When urine collection: after morning hygiene procedures taken midstream morning urine in an amount of 10-15 ml of a sterile dishes. Deliver to the laboratory within 2 hours.

When taking a smear from the throat and nose: can not be in the morning to brush your teeth, rinse your mouth and nose with a disinfectant solution, drink and eat.

fence feces It must be carried out in the morning sterile spatula into a sterile container in the amount of 15-30 grams. You can not allow in a urine sample. Delivery within 5 hours. Do not freeze or night deposit. Collect feces without the use of enemas and laxatives.

Blood for bacterial inoculation test taken prior to antibiotic treatment in an amount on the background in a sterile tube temperature rise not less than 5 ml (children) of at least 15 ml (adults).

Sputum It is going in the morning on an empty stomach in a sterile container during coughing with mucus. Before the fence brush your teeth and rinse your mouth with boiled water. Transported to the laboratory for 1 hour.

Breast milk It is going after hygienic procedures. Peripapillary area treated swab moistened with 70% ethanol. The first 15 ml of human milk is not used. Then, 5 ml is decanted into a sterile container. Deliver within 2 hours.

Separated genitals: women fence shall be held not earlier than 14 days after menstruation, not earlier than 1 month after discontinuation of antibiotics, preferably within 2 hours, unable to urinate; in men - it is not recommended to urinate for 5-6 hours prior to sampling.

Dates readiness bacteriological seeding

In the study of mucus from the nasopharynx results will be ready in 5-7 days, the study of bowel movements will take about 4-7 days.

On examination of the urogenital tract scraping length of the study will take 7 days. Seeding for the total flora lasts 4-7 days. Most of the duration of preparing blood for sterility - 10 days.

However, the earliest preliminary result can give within 3 days.

Result of bacteriological research

bacterial inoculation test result is a qualitative assessment of both (the mere presence of the pathogen in the sample) and the quantitative evaluation (concentration of agent in the material).

Deciphering the quantitative result is held in the simplest way. Allocate 4 growth degree (contamination) of microorganisms in the material. For 1 degree lean growth characteristic of growing in liquid medium only, on hard - no growth; 2 degrees - growth on solid medium to form single colonies 10; 3 degree - from 10 to 100 colonies; 4 degrees - more than 100 colonies.

It is important to conditionally pathogenic flora, the detection of which 1 and 2 degree is not considered a cause of the disease, it's just evidence of contamination of the material for the study, grade 3-4 indicate the etiology (cause) disease. If isolated pathogenic flora, that measure all the selected colonies, it has all the 4 degrees.

Result of counting colonies in CFU / ml is interpreted as follows: 103 / ml 1 colonies detection means; 104 / ml - from 1 to 5 colonies; 105 / ml - growth of colonies of 5-15; 106 / ml - greater than 15.

The quantitative result is important not only for determining the degree of contamination, but also ensure the accuracy of the treatment.

antibiotikogrammy

Determination of the sensitivity of the isolated microorganism to a particular antimicrobial drug is an important component of bacteriological research. Set products, which is sensitive or resistant pathogen, and is antibiotikogrammy.

antibiotikogrammy

The sensitivity of the pathogen - a susceptibility to a drug that is an antibiotic will affect the growth and reproduction of microbes. Resistance - resistance of the pathogen to a particular drug, that is an antibacterial drug will not work.

Antibiotikogrammy issued in specific units - minimum inhibitory concentration (MIC).

A source: https://medicalj.ru/diacrisis/d-immunology/588-bakposev

Bakposev sperm: indications, preparation, delivery, decoding

In today's world one of the most serious problems is male infertility. Since it is practically facing each fifth couple. To combat sterility requires accurate diagnosis.

One of the methods of recognition of the disease is considered to be a sperm bakposev. This analysis in 95% of cases can accurately determine the cause of the lack of children.

However, not only bacterial seeding semen is used to solve this problem.

What is semen?

This survey, which allows to evaluate the quality and quantity of the semen of men. Semen is considered to be one of the main methods of diagnosis of infertility. However, it can not be regarded as a kind of test for the possibility of conceiving a baby. Why?

The structure of sperm, their speed, and the total content of the ejaculate - are the key parameters that determine the likelihood of conceiving a child. Not always a good analysis ensures that man will soon become a father. About 30% of men suffering from infertility, know what semen, and have good results of the survey.

On the other hand, poor performance and should not be considered as a final verdict. To confirm the diagnosis of "infertility" the man must be a complete examination and pass several tests, including bakposev sperm. For more details about it will be discussed later.

Why this analysis?

Letting semen bacterial seeding is recommended for all men without exception, who have trouble conceiving a baby.

If the couple is preparing for a year to replenish, not using contraception, and pregnancy does not occur, you can talk about the problems with the reproductive system.

In this case, the examination for suspected infertility need to pass not only man, but also his companion.

Previously culprits to this problem were considered representative of the fair sex. Today, the statistics say otherwise. Stress, smoking, mental tension - all these factors affect the quality of sperm, as a consequence, the ability to have children.

Man has the potential to conceive a child, if it in 1 ml of ejaculate contains about 20 million sperm. When this figure is lowered to the level of 10 million or less, the chance of pregnancy is very small.

Even after ejaculation sperm for several hours to remain active. Otherwise, man is absolutely necessary to make bacterial seeding ejaculate.

In some cases, doctors prescribe more sperm bakposev?

1. Identifying the symptoms of inflammation genitals of infection.
2. The presence of prostatitis and related diseases.
3. Detection of pathologies, sexually transmitted diseases, with a partner.

This analysis reveals dermatological, urological and venereal problems have surveyed. With it, a physician can prescribe competent treatment. Sowing on flora gives a fairly accurate results.

Preparation for the examination

Delivery of the analysis - this important event, so approach it should be taken seriously. In addition, you must follow certain rules. Failure to comply with at least one of them is fraught with irregularities in the collection of biological material. Therefore, the results may be inaccurate.

Each clinic has its own rules of ejaculate fence. It is better to specify them in advance. However, there are general guidelines, compliance with which is very important.

1. 3-5 days before the delivery of sperm is necessary to refuse intimacy and other actions that may lead to ejaculation.
2. During the weeks before the analysis can not go to saunas and baths, drinking alcohol and smoking.
3. Collection of biological material must be carried out only in the sterile container. Some clinics provide a container for analysis. Others are allowed to buy it in pharmacy chains.

It should be noted that the capacity for collection of biological material must be new and clean. Never open up until the delivery of the analysis, touch the interior surface. Such actions can lead to the ingress of bacteria. As a result, the man received unreliable diagnosis.

How is the delivery of the analysis?

Before collecting biological material man should wash hands and genitals. It is prohibited to use antibacterial agents.

Generally, sperm are obtained by masturbation or stimulation ejaculatory center special electrodes. They are administered directly into the rectum.

During the collection of the material is important to ensure that sweat, lint from clothing or hair do not fall into the container for analysis. To pour it from one vessel to another is prohibited.

If the day before the man happened pollution survey, you must notify the doctor. Most likely, the need to shift the test date for a few days.

Doctors do not recommend to make a fence material at home. Unfortunately, not everyone is able to follow the above health requirements. For analysis is suitable only complete a portion of sperm. We must try not to lose it the first part, because that is where there is the main stream of micro-organisms.

Check ejaculate in the laboratory

Sperm were placed into an artificial environment which is considered favorable for the growth of microorganisms. For several days laboratory to maintain a certain temperature in a biological material containers.

Microorganisms are beginning to multiply and join the colony. They can be easily seen under a microscope. In professional duties includes counting the number of microorganisms, the rate of reproduction, detection sensitivity to various antibiotics.

A detailed study of the material subsequently allows the doctor to appoint a competent treatment.

Decoding results

Decrypt bakposev sperm can only qualified physician. Why? The thing is that the body of each person is different. It has its own characteristics. For one man the presence of certain micro-organisms in the semen is normal, but for another - artful diagnosis. Therefore, independently conduct transcripts analysis is not recommended.

Its execution time typically varies within 5-8 days. After that, a man takes form with the answers. In our time, the survey results can be obtained by phone or email.

cost analysis

What is the cost to deliver the semen analysis? Price survey depends on the clinic (private / public), the region in which it is located. The average cost of such services ranges from 500-1500 rubles.

The health of any person requires urgent attention. If you dream of replenishment in the family, but all attempts were in vain, it is necessary to pass the semen for analysis. Test necessarily help identify the cause of failure. However, only a qualified doctor under force find a solution to this problem as soon as possible.

9 famous women who fall in love with women Manifestation of no interest to the opposite sex is not something unusual. You can hardly surprise or shock someone, if we recognize that.

11 countries sign indicating that you are good in bed you also want to believe in what you deliver your romantic partner pleasure in bed? At least, you do not want to blush and apologize.

20 things you did not know about the movie "Pretty Woman" In 1990, screens out a favorite romantic comedy, which became an instant hit and has not lost its charm even after a quarter century. The film "Kras. 13 signs that you have the best husband Husbands - a truly great men. What a pity that a good wife does not grow on trees. If your significant other is doing these 13 things, you can with.

These 10 little things a man notices a woman always think your man knows nothing of the psychology of women? This is not true. From the look of loving your partner is not a single detail escapes. And here are 10 things.

Unforgivable mistakes in movies, which you probably never noticed I guess there are very few people who would not like to watch movies. However, even in the best movies are mistakes that can notice the spectators.

sources: http://fb.ru/article/263222/bakposev-spermyi-pokazaniya-podgotovka-sdacha-rasshifrovka

http://1lustiness.ru/ochishchenie-organizma/sovety/87405-bakposev-spermy-pokazaniya-podgotovka-sdacha-rasshifrovka

Sowing secret Helix Prostate - Treatment potnetsii

The prostate is an important organ in the male reproductive system. It produces a special mucus (secret), which contain the essential elements, immunoglobulins and many other useful vitamins.

Secret and its pathology

The substance produced during arousal and ejaculation, thereby enabling the sperm movement. In the development of the male cell, the prostate is involved in their origin. The secret, the right to study the tank. sowing gland, prepared by prostate massage.

Evaluation of prostate secretions make the presence of infectious diseases. This second analysis, allowing uzist determine the cause of prostatitis. Most often the tank. seeding is carried out in cases of suspected chronic inflammation of the prostate. When normal matter expert can detect individual bacteria, they are not dangerous and are not considered agents of pathological changes in the prostate gland.

Most often, when a secret study can be found bacteria such as:

• stafilokkok;
• klebsiely;
• Pseudomonas aeruginosa;
• mushrooms;
• enterobacteria.

The list is long, every foreign body, affecting the appearance of the disease, is considered to be specific. Bacterial prostatitis is usually seen gonococci and Trichomonas. The disease is then called gonorrhea and trichomoniasis.

In the study of the tank. seeding determined concentration of microbes, their type. Also, after the results of a doctor can prescribe appropriate treatment. In order to know what to prescribe antibiotics, a specialist conducting a survey found microorganisms and determine their development.

How to get the secret

To obtain material for study, do massage the prostate. Released fluid from a urinary canal is applied on a small glass is then sent to the laboratory.

Morning urine leaches a substance produced by the prostate, but for various reasons are not leaked. If the urine shows abnormalities, the doctor sees this as a change in the area of ​​the prostate gland, and appoint additional examinations.

In some cases, tests are taken after the patient taking alcohol or spicy foods. This method of obtaining the results called provocative. So it is possible to increase the blood flow to the prostate. The resulting material is output to a medium, and then determine the type of tank. exciter.

Direction on the procedure for obtaining the material for the study from a urologist. He will give the necessary guidance and decipher the results.

Preparation for Analysis and its regulations

Some training seeding prostate secretion is not required. Experts recommend to refrain from sexual intimacy for 24 hours before putting the secret. There are also items that are best followed in order to obtain correct and precise indicators. It is such things as:

• break in antibiotics (for a few days before the procedure should stop taking the medication);
• rejection of alcohol and spicy food;
• exception of physical activity, fatigue;
• enema (recommended by doctor).

When healthy prostate secret number may be up to 2 mL. When deviations volume of the substance is reduced, and at a standstill - increases. The color of the secret should be with a light shade in the pathology he gets a yellow or pink.

• If the obtained first treatment tank. sowing leukocytes are found within the normal range, it does not mean that there is no inflammation. I am sure after some time it is necessary to pass the secret on the tank. crop again.
• The smell of slime like the smell of spermine, it can be any case of deviations. Density has a secret number 1022 (no more and no less). Any jump of this figure is regarded as a pathology.
• Hydrogen parameter in a rate of from 6.4 to 7 (neutral and acidic medium). If there is a chronic prostatitis - an alkaline environment will, in the case of acute inflammation - sour.
• Red blood cells, mitrofagi, amyloid corpuscles, large cells, fungi - are absent or occur in single quantities. All of these elements appear in the inflammatory processes of urogenital system in men.
• Epithelial cells discern in the number of 1-2 pieces. If the index increased at least by one unit, this indicates an inflamed prostate.
• Lecithin grains should be in a large amount (more than 10 Mill.). If there is inflammation of the prostate, the figure is sharply reduced.

When it is not necessary to do an analysis on bacterial seeding

Despite the simple manipulation to obtain secretion of the prostate, this method of study has its own restrictions. Contraindicated in patients with the following pathological processes:

• cracks in the anus;
• elevated temperature (from 38 degrees or higher);
• current acute disease, inflammation;
• all kinds of hemorrhoids;
• Tuberculosis of the prostate.

If there is at least one of these items, it is better to pass an examination ejaculate, which will also feature the presence of infectious processes in the urogenital system.

Infertility - a problem which faced virtually every 4-5 a married couple now. And in about 45-50% of the blame for the fact that there is no conception, it is a man. Determine the cause, because of which it is impossible to conceive by natural means, - the main task of the doctors involved in the treatment of male infertility.

And one of the most important steps in the fight against disease is a competent and deep diagnostics. One of the most common diagnostic techniques is sowing ejaculate. This analysis, in most cases, the most accurate method to determine the cause of male infertility.

In addition, the seeding of the ejaculate is appointed for men in the presence of suspected infectious diseases, inflammations or other disruption of the male urogenital system. Many men assigned not only the sowing of ejaculate, but other diagnostic measures.

Why the analysis tank sowing?

Bacteriological analysis of ejaculate allows Andrology and Urology to obtain valuable information on the state of health of the patient. In the analysis of micro-organisms found sowing, due to which the inflammatory process has begun in the male reproductive system.

This is particularly important for maintaining sexual health of men, that is, its ability to continue the race.

Timely delivery of seeding tank allows time to detect infections that are sexually transmitted, allowing the man to avoid possible complications and avoid infecting their sexual partner.

Letting ejaculate on bacteriological seeding should be done in almost all situations where there are symptoms of inflammation male genital organs. These symptoms may be the clinical and laboratory.

The clinical signs are manifested as pain, burning, various discharge with an unpleasant odor and contaminants. Among the laboratory signs include, for example, increased the proportion of leukocytes in the ejaculate analyzed.

Thus, the bacteriological seeding is assigned in the following situations:

• for suspected male sterility;
• in the presence of infectious diseases and inflammatory processes of the male reproductive system;
• for suspected prostatitis and a number of other diseases;
• in the presence of a woman, which had examinee male sex, disease, sexually transmitted diseases.

Thus, the bacteriological seeding is a very important analysis, the most accurate detecting pathogens a number of diseases in urology, dermatology, Venereology and other areas medicine.

A study of male microflora conducted on this technique, considered to be one of the most accurate, sensitive and specific.

When taking on the ejaculate analysis must comply with a number of important conditions on which performance depends on the accuracy of the test result.

How is the delivery of semen for analysis?

There are a number of approved methods that are used when taking ejaculate on bacteriological seeding.

Typically, it is obtained by masturbation or the stimulation ejaculatory Center using special electrodes introduced into the rectum. Chance of vibro penis.

It is important to tell the specialist who will carry out the analysis of the results of decoding, all transferred diseases and medications taken, because many of them can lead to a significant distortions.

As a rule, the ejaculate is collected in a special sterile container with a wide mouth. In addition, the assay can be used postcoital sperm.

In this case, its collection is carried out without a condom lubricants and spermicides. The thus obtained ejaculate to be delivered on study for 1 hour.

When transporting the material must be at a temperature corresponding to body temperature.

The analysis may be conducted using postorgazmennoy incontinence, retrograde ejaculation, when sperm is thrown into the bladder through nesomknuty sphincter.

Such a procedure is usually assigned in the absence or too low volume of ejaculate.

In this case, bladder catheterization is performed, in which are special environment, or alkalinisation of urine, which allows to preserve the conditions of sperm activity.

The optimal and widely used method of sampling is a sperm masturbation.

Terms of putting semen on bakposev

Before any semen for analysis need to wash your hands and genitals. From the last sexual intercourse before going to the hospital should go 3-5 days.

During this time, you need to refrain from visiting the baths and drinking. If possible (necessarily agreeing with your doctor) should stop taking the drugs.

Massage is contraindicated in the seminal vesicles and prostate. Deliver sperm received at home is unacceptable.

Analyzed can only complete a portion of the ejaculate, as in its various parts contain a different number of spermatozoa and other microorganisms. We need to try not to lose the first portion of the ejaculate.

It was established that it contained about 75% of the total sperm count.

Those sperm that are present in the remaining volume, usually have a lower mobility, which may distort the results of the analysis if it surrenders to determine the cause of infertility.

Sperm is collected in a clean and dry glass vessel, which had previously heated to body temperature. The material must be delivered to the study no later than 3 hours after the intake. If sperm can not be delivered within the specified time, until the moment of delivery it must be stored in the refrigerator, but no longer than 1 day.

If planting is carried out for bacteriological research and myco-ureaplasma, the ejaculate is placed in a special bottle with a medium for mycoplasma. There is also a medium for cultivation of Trichomonas. When the direction of the doctor must tell the patient that he warned lab workers.

How is the analysis?

Sperm placed in a special culture medium favorable to the "growing" of various microorganisms. A few days in the medium to maintain a certain temperature.

During the multiplication of micro-organisms together in colonies, which are distinguishable under a microscope. Specialist conducting analysis determines the presence and amount of microorganisms, their reproduction rate, sensitivity to various antibiotics.

This allows you to choose the most effective treatment program.

Within 5-8 days the identification of microorganisms and antibiogram. When everything is ready, the patient receives at the hands of the form with the results of bacteriological seeding. Modern laboratories provide results by telephone, e-mail, online labs, etc.

Microbiologist conducts decoding bacterial inoculation test. Finally, the doctor receives. If bad analysis, the man appointed by the appropriate treatment.

This analysis allows us to evaluate the role of microorganisms in the development of an infectious or inflammatory process. It can be used to establish the degree of conditionally pathogenic microorganisms. Only after that can be treated with antibiotics and other drugs.

There permissible limits the presence of certain organisms in which they do not lead to the development of diseases. For instance, mycoplasma and ureaplasma are representative of the normal human flora and lead to the development disease only in those cases where too start to actively proliferate under the influence of certain factors.

In medicine, there is such a thing as colony-forming units. It is understood as a microbial cell that later grows into a colony. In this unit, and the results of bacteriological seeding.

Each seeding method has established standards, indicating a clinically significant concentration of bacteria that can lead to the development of the disease.

If the colony-forming units exceed the permissible limits, the analysis is considered bad. Obligatory in the study material and checked the sensitivity of microorganisms to antibiotics.

This analysis allows you to assign the most rational antibiotic therapy.

A source: http://dieta.lechenie-potencya.ru/prostata/posev-sekreta-prostaty-heliks/